Effect of sensitization on spontaneous and phosphatidylserine-induced histamine release and on cyclic AMP and GMP levels in isolated rat mast cells
- 1 August 1979
- journal article
- research article
- Published by Wiley in Acta Physiologica Scandinavica
- Vol. 106 (4) , 473-479
- https://doi.org/10.1111/j.1748-1716.1979.tb06428.x
Abstract
Sprague Dawley rats were sensitized with 20 .mu.g or 100 mg egg albumin (using pertussis vaccine as adjuvant). Mast cells isolated from the former group of animals showed a higher degree of histamine release upon challenge in vitro with egg albumin than those from the latter group. Using the lower amount of antigen for immunization mast cells from Hooded Lister rats showed an even higher degree of histamine release induced by antigen. An increased antigen-induced histamine release was associated with an increased spontaneous and phosphatidylserine-induced histamine release. Histamine release induced by phosphatidylserine was specific in so far as it was Ca dependent and theophylline-inhibited. The basal level of cyclic[c] AMP in mast cells was significantly depressed by sensitization. There was a relationship between the cAMP/GMP ratio and the degree of spontaneous, phosphatidylserine-induced and anaphylactic histamine release. Sensitization induces an increased release of histamine not only to the specific antigenic stimulus but also to more unspecific stimuli. Concomitantly there is a fall in the cAMP/cGMP ratio. The relationship between these 2 phenomena is discussed.Keywords
This publication has 26 references indexed in Scilit:
- Relationship between serum IgE levels and anaphylactic histamine release from isolated rat mast cellsActa Physiologica Scandinavica, 1979
- Human lung tissue and anaphylaxisJournal of Allergy and Clinical Immunology, 1977
- Changes in ATP and Cyclic Nucleotide Levels during Sympathetic Nerve Stimulation in Canine Subcutaneous Adipose Tissue in situActa Physiologica Scandinavica, 1977
- Release of prostaglandins and histamine from sensitized and anaphylactic guinea pig lungs—changes in cyclic AMP levelsBiochemical Pharmacology, 1977
- Cyclic AMP independent inhibition by papaverine of histamine release induced by compound 48/80Biochemical Pharmacology, 1976
- Potentiation of Anaphylactic Histamine Release from Isolated Rat Pleural Mast Cells by Rat Serum PhospholipidsActa Physiologica Scandinavica, 1976
- Storage of 5‐Hydroxytryptamine in Rat Mast Cells. Evidence for an Ionic Binding to Carboxyl Groups in a Granule Heparin‐Protein ComplexActa Physiologica Scandinavica, 1972
- Phosphatidylserine: Selective Enhancer of Histamine ReleaseScience, 1971
- Antigenic Histamine Release from Fractionated and Unfractionated Peritoneal Cells from Sensitized RatsActa Pharmacologica et Toxicologica, 1968
- Degranulation and Histamine Release, Two Consecutive Steps in the Response of Rat Mast Cells to Compound 48/80Acta Physiologica Scandinavica, 1967