URINARY EXCRETION OF 17-KETOSTEROIDS FOLLOWING RECTAL INFUSION OF CORTISOL

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Abstract
Urinary 17-ketosteroid excretion patterns were compared, following rectal and oral administration of cortisol to a man with quiescent ulcerative colitis. Steroid conjugates extracted from the urine were hydrolyzed to yield glucuronide and sulphate fractions, and the free steroids separated on alumina. Individual compounds were identified by paper chromatog-raphy and by infrared spectroscopy. Rectal infusions of cortisol produced a 100-fold increase in urinary 11[beta]-hydroxyetiocholanolone excretion (from 92 [mu]g. to 10,350 [mu]g. daily), and a 25-fold increase in 11[beta]-hydroxyandrosterone excretion (from 98 ug. to 2465 [mu]g. daily). Other 17-ketosteroids were hardly changed. Oral cortisol had little effect on urinary 17-ketosteroid excretion. The increased excretion of 11[beta] -hydroxyetiocholanolone and 11[beta] -hydroxyandrosterone after rectal infusion of cortisol did not occur when the bacterial activity of the gut was reduced by giving neomycin orally.