The SH-group content of human foetal and sickle-cell haemoglobins

Publisher Website
Google Scholar
Abstract
The SH-group contents of human fetal and sickle-cell hemoglobins have been determined by amperometric titration with phenyl mercuric hydroxide. Some variation was found in the SH-group content of different samples of fetal hemoglobin. The mean values from 6 samples were 20 SH groups per mole in the native protein and 4.2 SH groups per mole in the denatured protein. Possible reasons for this variation are discussed. Sickle-cell hemoglobin had the same SH-group content as normal human adult hemoglobin, namely 2.2 SH groups per mole in the native protein and 6.0 SH groups per mole in the denatured protein.