Studies on poison ivy.

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Abstract
The isolation and purification of poison ivy urushiol is described. The preparation of urushiol-ski protein and urushiol human serum albumin is also described. Lymphocytes from eleven donoi naturally sensitized to poison ivy and from four non-sensitive individuals have been cultured fo 5 days in the presence of urushiol-carrier conjugates. Lymphocytes from seven of the eleven sensitive, donors responded with a stimulation index greater than 3.0 to urushiol-albumin conjugate. When urushiol-skin protein conjugate was used as a stimulant, lymphocytes from only three of the eleven sensitive donors responded. The results suggest that urushiol-protein conjugates can stimulate sensitive lymphocytes in vitro, although a response is not observed in every individual naturally sensitized to poison ivy.