Prolonged Storage of Marrow and Its Use in the Treatment of Radiation Injury

Abstract
Marrow was removed from the femurs of dogs and frozen in 15 per cent glycerol at 1 C. per minute. Marrow from one dog was stored at −80 C. and from the other at −180 C. for a period of 14 months. Each dog was then given 1,200 r of whole body irradiation followed by infusion of his own thawed marrow. Recovery was rapid, and the dogs were living and well two months later.