Rapid, low-input, low-bias construction of shotgun fragment libraries by high-density in vitro transposition
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Open Access
- 8 December 2010
- journal article
- research article
- Published by Springer Nature in Genome Biology
- Vol. 11 (12) , 1-17
- https://doi.org/10.1186/gb-2010-11-12-r119
Abstract
We characterize and extend a highly efficient method for constructing shotgun fragment libraries in which transposase catalyzes in vitro DNA fragmentation and adaptor incorporation simultaneously. We apply this method to sequencing a human genome and find that coverage biases are comparable to those of conventional protocols. We also extend its capabilities by developing protocols for sub-nanogram library construction, exome capture from 50 ng of input DNA, PCR-free and colony PCR library construction, and 96-plex sample indexing.Keywords
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