Enzyme-linked immunosorbent assay for the detection of serum antibody to outer membrane proteins of Treponema pallidum.

Publisher Website
Google Scholar
Abstract
A highly sensitive enzyme-linked immunosorbent assay was used for the analysis of serum IgG reactivity against specific immunogenic T. pallidum proteins. Outer membrane treponemal proteins purified by preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis were used as antigenic probes at concentrations as low as 100 ng/ml (5 ng/well in microtiter plates). Detection of anti-treponemal antibody was possible using rabbit syphilitic sera diluted to 1/10,000. The sensitivity of the assay was equal to or greater than that detected by radioimmunoprecipitation combined with gel electrophoresis and fluorography techniques and was capable of monitoring host IgG responses throughout the progress of the disease.