Sequence analysis of hepatitis A virus cDNA coding for capsid proteins and RNA polymerase.
- 1 April 1985
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 82 (7) , 2143-2147
- https://doi.org/10.1073/pnas.82.7.2143
Abstract
The nucleotide sequence corresponding to 2 large regions of the hepatitis A virus (HAV) genome is reported. These comprise a sequence of 3274 bases corresponding to the 5'' end of the genome, which includes the putative capsid protein region of this picornavirus, and 1590 bases corresponding to the 3'' end of the genome, terminating in a 15-base poly(A) tract. These sequences revealed that HAV had the characteristic genomic organization of picornaviruses: an open reading frame beginning .apprx. 750 bases from the 5'' end of the RNA and a termination codon 60 bases from the 3'' poly(A) tract. The predicted amino acid sequences of both regions were compared to analogous regions previously determined for other picornaviruses. There was sufficient homology to conclude that the 5'' region of HAV codes for capsid proteins and that the 3'' region codes for an RNA polymerase. These regions of HAV were not found to be closely related to analogous regions of poliovirus, encephalomyocarditis virus and foot and mouth disease virus.This publication has 26 references indexed in Scilit:
- Relationship of human rhinovirus strain 2 and poliovirus as indicated by comparison of the polymerase gene regionsVirology, 1984
- Complete nucleotide sequences of all three poliovirus serotype genomesJournal of Molecular Biology, 1984
- Molecular cloning and characterization of hepatitis A virus cDNA.Proceedings of the National Academy of Sciences, 1983
- Identification of amino acid and nucleotide sequence of thefoot-and-mouth disease virus RNA polymeraseVirology, 1983
- The nucleotide sequence of cDNA coding for the structural proteins of foot-and-mouth disease virusGene, 1982
- Cloned Poliovirus Complementary DNA Is Infectious in Mammalian CellsScience, 1981
- Primary structure, gene organization and polypeptide expression of poliovirus RNANature, 1981
- 3′-End labeling of DNA with [α-32P] cordycepin-5′-triphosphateGene, 1980
- BK virus DNA sequence coding for the amino-terminus of the T-antigenVirology, 1979
- A new method for sequencing DNA.Proceedings of the National Academy of Sciences, 1977