High tyrosine protein kinase activities in soluble and particulate fractions in bone marrow cells
Open Access
- 7 May 1984
- journal article
- Published by Wiley in FEBS Letters
- Vol. 170 (1) , 139-142
- https://doi.org/10.1016/0014-5793(84)81386-1
Abstract
High tyrosine protein kinase activities were detected in soluble and particulate forms from bone marrow cells using synthetic peptide (Glu‐Asp‐Ala‐Glu‐Tyr‐Ala‐Ala‐Arg‐Arg‐Arg‐Gly) as a substrate. Total activity of tyrosine protein kinase was 2.4 times higher in the soluble fraction but the specific activity was slightly higher in the particulate one. Mg2+ or Mn2+ requirements of these two enzymes for maximal activity were quite different from each other. Physiological significance of these two forms of enzymes is briefly discussed.Keywords
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