Cocarboxylase and Thiamine in Tissues of Rats Receiving Different Concentrations of Vitamin B1 in the Diet

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Abstract
The influence of the concentration of vitamin B1 in purified diets upon rat tissue concentrations of cocarboxylase and thiamine has been demonstrated. As the dietary concentration of vitamin B1 was increased up to 0.2 mg per 100 gm of diet, the concentration of cocarboxylase also increased in brain, muscle and liver. However, a dietary concentration of vitamin B1 greater than 0.2 mg per 100 gm of diet gave no further increase in tissue cocarboxylase. In contrast, as the dietary concentration of vitamin B1 was increased to 0.4 mg per 100 gm of diet, the tissue concentration of thiamine also increased, except in the brain. The vitamin B1 requirement for rat growth was compared with the dietary vitamin B1 concentration necessary to give maximum tissue concentrations of cocarboxylase and thiamine. In agreement with other workers, about 0.1 mg of vitamin B1 per 100 gm of diet was required to support normal growth. However, at least 0.2 mg of vitamin B1 per 100 gm of diet were required to give maximum cocarboxylase levels, and tissue thiamine concentrations increased up to 0.4 mg of vitamin B1 per 100 gm of diet, except in brain tissue.