Human somatotropin: biological characterization of the recombinant molecule.
- 1 October 1976
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 73 (10) , 3463-3465
- https://doi.org/10.1073/pnas.73.10.3463
Abstract
The recombinant hormone obtained by non-covalent interaction of the NH2-terminal 134 amino acid fragment with the COOH-terminal 51 amino acid fragment of the reduced-carbamidomethylated human somatotropin molecule is found to exhibit nearly full biological activity of the native hormone, as evidenced by the stimulation of hepatic ornithine decarboxylase (L-ornithine carboxy-lyase, EC 4.1.1.17) in vivo and protein synthesis in mouse mammary gland in vitro. Radioimmunoassay data indicate that the recombinant behaves immunochemically in a manner almost identical to that of the native hormone.Keywords
This publication has 11 references indexed in Scilit:
- Human pituitary growth hormone: restoration of full biological activity by noncovalent interaction of two fragments of the hormone.Proceedings of the National Academy of Sciences, 1976
- Human Pituitary Growth Hormone. 44. Effects of Plasmin-Modified Hormone and Its Fragments on Ornithine Decarboxylase Activity and LipolysisExperimental Biology and Medicine, 1976
- Isolation and Biological Characterization of Fragments of Human Growth Hormone Produced by Digestion with PlasminEndocrinology, 1975
- Human Pituitary Growth Hormone Isolation and Properties of Two Biologically Active Fragments from Plasmin DigestsProceedings of the National Academy of Sciences, 1974
- Metabolic Effects of Plasmin Digests of Human Growth Hormone in the Rat and ManJournal of Clinical Investigation, 1973
- Isohormones of Human Growth Hormone. II. Plasmin-Catalyzed Transformation and Increase in Prolactin Biological Activity*Endocrinology, 1973
- On the Purification of l-Ornithine Decarboxylase from Rat Prostate and Effects of Thiol Compounds on the EnzymeJournal of Biological Chemistry, 1971
- Plasma Growth Hormone Concentration in the Rat Determined by Radioimmunoassay: Influence of Sex, Pregnancy, Lactation, Anesthesia, Hypophysectomy and Extrasellar Pituitary TransplantsEndocrinology, 1966
- THE PREPARATION OF 131I-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITYBiochemical Journal, 1963