Purification and Some Properties ofβ-Fructofuranosidase I from Arthrobacter sp. K-1

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Abstract
Arthrobacter sp. K-1, isolated from soil, produces β-fructofuranosidase. This enzyme preparation was separated into three fractions (I, II, and III) by butyl-Toyopearl 650 M column chromatography. The β-fructofuranosidase I was purified to homogeneity by disc-electrophoresis after consecutive column Chromatographies. The enzyme had a molecular weight of 52,000 by SDS-polyacrylamide gel electrophoresis and 51,000 by gel filtration with Ultrogel AcA 44, and an isoelectric point of 4.3. The enzyme was most active at pH 6.5–6.8 and at 55°C and stable up to 45°C at pH 6.5 for 30 min of incubation, and from pH 5.5 to 10.0 at 40°C in 2 hr of incubation. This enzyme hydrolyzed sucrose, erlose, raffinose, fructosylxyloside, neokestose, and stachyose at the relative velocities of 100, 75, 61, 59, 54, and 11, but hardly hydrolyzed 1-kestose and nystose (<0.02).