The Use of Low Ionic Strength Solution (LISS) in Elution Experiments and in Combination with Papain‐Treated Cells for the Titration of Various Antibodies, Including Eluted Antibody

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Abstract
This investigation demonstrates the value of suspending [human] red cells in a LISS for elution experiments since this has the effect of increasing the uptake of antibodies by the red cells. For the titration of low affinity antibodies, and also of eluted antibodies, the combining capacity of which may be impaired through the elution process, it is an advantage to use a LISS-enzyme (papain) technique.