Separation and Assignment of the Tryptic Peptides of Human Growth Hormone (hGH) and the 20K Dalton hGH Variant by Reversed Phase High Performance Liquid Chromatography
- 1 January 1983
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography
- Vol. 6 (2) , 215-228
- https://doi.org/10.1080/01483918308066884
Abstract
Reversed-phase high performance liquid chromatography (RP-HPLC) has been used to separate the peptides generated by tryptic cleavage of human growth hormone (hGH) and the 20K dalton human growth hormone variant. The total amino acid compositions of both these pituitary proteins has been accounted for on the basis of these chromatographic mapping procedures. Structural analyses of the peptides isolated from semi-preparative RP-HPLC separations has confirmed that the primary structure of the variant differs from that of the sequence of hGH by deletion of the amino acid residues 32–46.This publication has 19 references indexed in Scilit:
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