Identification of guanosine triphosphate as the 5'-terminus of RNA from bacteriophage Q-beta and R23.
- 1 February 1968
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 59 (2) , 513-518
- https://doi.org/10.1073/pnas.59.2.513
Abstract
Alkaline hydrolysis of RNA from the RNA phages Q[beta]and R23 yielded a compound identified as guanosine 5[image]-tri, 2[image],3[image]-monophosphate (pppGp). It was present in a 1:1 molar ratio with RNA labeled either with H3-guanine or p32 and was detected in experiments with bases specifically labeled only with H3-guanine. The electrophoretic mobility of the compound was identical to that of authentic p32ppGp both at pH 3.5 and 4.0. The degradation products guanosine and guanosine 3[image],5[image]-diphosphate plus guanosine 5[image]-monophosphate were obtained after treatment with alkaline phosphatase or after mild acid hydrolysis at pH 4.0, respectively.This publication has 12 references indexed in Scilit:
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