Physiological and Hormonal Factors Influencing Organogenesis in Rudbeckia bicolor Explants Cultured In vitro

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Abstract
Factors influencing organogenetic responses and bolting of adventitiously formed buds were investigated in in vitro cultured cotyledon, stem and leaf explants of Rudbeckia bicolor. Application of naphthaleneacetic acid (NAA) induced adventitious root formation and that of benzyladenine (BA) induced adventitious bud differentiation. When NAA at a low concentration was added together with BA, bud initiation and development were promoted further, although optimal concentrations of NAA and BA varied with the kind of explants used. Gibberellic acid caused stem elongation of adventitious buds, and occasionally differentiation of floral buds on the apices of developed shoots. The action of N-phenyl-N'-(4-pyridyl)urea (4PU) and its derivative (4PU-Cl) on adventitious bud formation was also examined.