The chemical synthesis of oligoribonucleotides. IX. A comparison of protecting groups in the dichloridite procedure
- 1 December 1980
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Chemistry
- Vol. 58 (23) , 2686-2693
- https://doi.org/10.1139/v80-428
Abstract
A series of phosphorodichloridites bas been prepared incorporating the most commonly used phosphate protecting groups in oligonucleotide synthesis. The groups include trichloroethyl, tribromoethyl, cyanoethyl, benzyl, methyl, p-chlorophenyl, and nitrophenethyl. The trichloroethyl and the nitrophenethyl appear to be the most stable groups while the cyanoethyl and methyl offer specific advantages. The benzyl and p-chlorophenyl groups are subject to limitations on their utility. Condensations can be carried out in a range of solvents including THF, pyridine, and DMF and at temperatures from −78 °C to 20 °C with a slight drop in yield with increasing temperature, at least for dinucleotide condensations.This publication has 6 references indexed in Scilit:
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