Diagnosis of Acute Acquired Toxoplasmosis with the Enzyme-Labelled Antigen Reversed Immunoassay for Immunoglobulin M Antibodies

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Abstract
The reverse enzyme-labeled antigen immunoassay (R-EIA), based on the capture of serum IgM and subsequent addition of Toxoplasma gondii soluble antigen tagged with peroxidase and substrate, was evaluated comparatively with the IgM-indirect immunofluorescence test (IgM-IIF) for the detection of antitoxoplasma IgM antibodies in sera from individuals with diagnosed acute acquired toxoplasmosis. Additional serum groups from normal healthy individuals and sera presenting possible nonspecific reactivities were also evaluated. Complete specificity of R-EIA was shown. There was no correlation between the magnitude of R-EIA results and IgM-IIF titers, but a positive (although not linear) correlation was found between R-EIA and the IgM-IIF titers obtained after adsorption of sera with Staphylococcus aureus protein A. Direct labeling of the antigen by a simple coupling technique facilitated the assay standardization and improved its signal-to-noise ratio.