Effect of Germination on Vitamin B12 Values of Pulses (Leguminous Seeds)

Abstract
A method of extraction of vitamin B12 from pulses has been described which will release a maximum amount of the vitamin. The vitamin B12 values of 7 varieties of pulses have been determined microbiologically using Lactobacillus leichmannii both before and after germination for 48 or 96 hours. In all of the pulses vitamin B12 values increased after germination for 48 hours and in most of the cases the increase was highest after 96 hours' germination.