Enzyme Immunoassay of Human Urinary Kallikrein

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Abstract
An enzyme immunoassay for the determination of human urinary kallikrein was developed and is compared with other human urinary kallikrein assays such as radioimmunoassay, dog blood pressure assay, rat uterus test after kinin liberation and synthetic substrate tests (AcPheArgOEt [N.alpha.-acetyl-L-phenylalanyl-L-arginine ethyl ester] and S-2266 [D-valyl-L-leucyl-L-arginine-p-nitroanilide]). The usable range of the standard curve is from 0.05 to 12 ng kallikrein per test. The intrassay coefficient of variation is between 2 and 4%, the interassay coefficient of variation is betweeen 4 and 12% and the recovery of authentic kallikrein added to urine samples is 95%. Human saliva and human pancreatic kallikrein show the same binding curves as purified human urinary kallikrein. Kallikrein from urine of rats, dogs and rabbits as well as boar acrosin and pig pancreatic kallikrein, bovine trypsin and chymotrypsin show no cross-reactivity.

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