Poliomyelitis Viruses in Tissue Culture. IV. Protein-Free Nutrient Media in Stationary and Roller Tube Cultures.

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Abstract
Poliomyelitis virus may be propagated in test tube, plasma-clot cultures of monkey testicular tissue in the absence of protein in the fluid phase of the nutrient medium. The tubes may be held stationary with the fragments submerged under a thin layer of medium, or they may be turned in a roller drum. The medium used contained lactalbumin enzymatic hydrolysate, serum ultra-filtrate, and balanced salt solution. With such a medium, fibro-blasts grow out within 4 days after the cultures are made, and are then suitable for use in virus titrations and neutralization tests in which the presence of virus is indicated by its cytopatho-genic effect on the fibroblasts. Also such cultures continue to produce virus for a period of about four weeks, long after the degeneration of the fibroblasts.