Complement Fixation with Brunhilde and Lansing Poliomyelitis Viruses Propagated in Tissue Culture.

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Abstract
Antigens which gave specific complement fixation were concd, from the supernatant fluids of tissue cultures infected with poliomyelitis viruses (Lansing and Brunhilde strains) by a method of ultrafiltration. Their anticomplementary activity was abolished by heating. The drop method of Fulton and Dumbell was used and offered a practical way of performing the tests with minimal amts. of antigen. Specific antibodies were demonstrated in the sera of hyperimmunized monkeys as well as in sera of poliomyelitis patients.