Protein Unfolding and Refolding Under Force: Methodologies for Nanomechanics
- 7 January 2005
- journal article
- review article
- Published by Wiley in Chemphyschem
- Vol. 6 (1) , 29-34
- https://doi.org/10.1002/cphc.200400343
Abstract
An increasing number of inter‐ and intramolecular interactions can nowadays be probed using single‐molecule manipulation techniques. Protein unfolding and refolding is the most representative—though complex—of these interactions. Herein, we review the main modes of performing a force unfolding experiment: the velocity clamp and the new force clamp mode. We also compare some of the physical aspects behind the two most frequently used single‐molecule manipulation instrumentations: optical tweezers and atomic force microscopes.Keywords
This publication has 33 references indexed in Scilit:
- Force-Clamp Spectroscopy Monitors the Folding Trajectory of a Single ProteinScience, 2004
- A mechanical unfolding intermediate in an actin-crosslinking proteinNature Structural & Molecular Biology, 2003
- Finding a protein's Achilles heelNature Structural & Molecular Biology, 2003
- Molecular nanosprings in spider capture-silk threadsNature Materials, 2003
- Hidden complexity in the mechanical properties of titinNature, 2003
- The myosin coiled-coil is a truly elastic protein structureNature Materials, 2002
- Reverse engineering of the giant muscle protein titinNature, 2002
- Reversible Unfolding of Single RNA Molecules by Mechanical ForceScience, 2001
- Overstretching B-DNA: The Elastic Response of Individual Double-Stranded and Single-Stranded DNA MoleculesScience, 1996
- Adhesion Forces Between Individual Ligand-Receptor PairsScience, 1994