Structural and Functional Differences of the Anionic and Cationic Antigens in K99 Extracts of Escherichia coli B41
- 1 July 1982
- journal article
- research article
- Published by Microbiology Society in Microbiology
- Vol. 128 (7) , 1585-1589
- https://doi.org/10.1099/00221287-128-7-1585
Abstract
Radiolabeled anionic and cationic components were purified from K99 extracts of E. coli B41 by immunoelectrophoresis using absorbed K99 antisera. SDS[sodium dodecyl sulfate]-polyacrylamide gel electrophoresis revealed that the apparent MW of the polypeptide subunits were 34,000 and 19,000, respectively. Both anionic and cationic antigens in cell-free K99 extracts adhered to sheep erythrocytes after 90 min at 4.degree. C, but the cationic antigen eluted after a further 1 h at 37.degree. C. The anionic antigen did not elute from sheep erythrocytes after 18 h at 37, 43 or 56.degree. C.This publication has 6 references indexed in Scilit:
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