Organization and Sequences ofp-Hydroxybenzaldehyde Dehydrogenase and Other Plasmid-Encoded Genes for Early Enzymes of thep-Cresol Degradative Pathway inPseudomonas PutidaNCIMB 9866 and 9869
- 1 January 1999
- journal article
- research article
- Published by Taylor & Francis in DNA Sequence
- Vol. 10 (1) , 7-17
- https://doi.org/10.3109/10425179909033930
Abstract
The gene (designated pchA) encoding the aldehyde dehydrogenase that is required to metabolise the p-hydroxybenzaldehyde produced by the degradation of p-cresol in Pseudomonas putida NCIMB 9866 and 9869 has been identified on plasmids pRA4000 and pRASOO, respectively. The gene lies immediately upstream of the pchC and pchF genes encoding the subunits of p-cresol methylhydroxylase (PCMH), the preceeding enzyme in the p-cresol degradative pathway. In pRA500 the latter genes are followed by the genes encoding the α (pcaG) and β (pcaH) subunits of protocatechuate-3,4-dioxygenase, whereas in pRA4000 the genes encoding PCMH are followed by an open reading frame encoding a protein that is similar to the maturase-related protein of P. alcaligenes. A gene, designated pchX, that encodes a protein of unknown function was identified between the pchC and pchF genes in both plasmids.Keywords
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