Abstract
17 laboratories situated in the U. S. and Canada have collaborated in a study of the utility of lauryl sulfate tryptose broth for detection of the presence of coliform organisms in water. 605 water samples from which primary gas positives were obtained, were examined, employing L.S.T. broth as a primary and as a secondary medium for comparison with lactose broth in the Standard Methods "completed test" procedure. Substitution of L.S.T. broth for lactose broth in the Standard Methods procedures promises a reduction in the number of primary gas positives to be confirmed and an increase in the number of positive indications of the presence of coliform organisms.

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