Simultaneous Determination of Cephalexin and Carbocisteine from Capsules by Reverse Phase High Performance Liquid Chromatography (RP - HPLC).
- 1 February 1997
- journal article
- pharmaceutical analysis
- Published by Taylor & Francis in Analytical Letters
- Vol. 30 (4) , 821-831
- https://doi.org/10.1080/00032719708006427
Abstract
A new, simple, precise, accurate and rapid RP- HPLC method has been developed for the simultaneous determination of Cephalexin and Carbocisteine from capsules, using 0.025 M monobasic sodium phosphate: acetonitrile (87:13, v/v) as a mobile phase, and a C8 Shodex column as the stationary phase. Detection was carried out using a UV detector at 210 nm. The linearity range for Cephalexin and Carbocisteine were 50 - 300 μg/mL and 40 - 200 μg/mL, respectively. Assay values for Cephalexin and Carbocisteine were 99.46% (R.S.D. - 1.30%) and 99.22% (R.S.D.-1.34%) for Brand 1. and 99.71% (R.S.D. - 1.68%) and 99.43% (R.S.D. - 1.78%) for Brand 2, respectively.Keywords
This publication has 11 references indexed in Scilit:
- Determination of S-carboxymethylcysteine in serum by reversed-phase ion-pair liquid chromatography with column switching following pre-column derivatization with o-phthalaldehydeJournal of Chromatography B: Biomedical Sciences and Applications, 1988
- An improved method for the determination of S-Carboxymethyl-L-cysteine in biological fluids with precolumn derivatization and on-line clean-upChromatographia, 1988
- Isotachophoretic analysis of amoxycillin and carboxymethylcysteine in pharmaceuticalsJournal of Chromatography A, 1987
- Quantitation of Cephalexin in Pharmaceutical Dosage Forms Using High-Performance Liquid ChromatographyDrug Development and Industrial Pharmacy, 1987
- Determination of cephalosporins utilising thin-layer chromatography with fluorescamine detectionThe Analyst, 1985
- Determination of ampicillin, amoxicillin, cephalexin, and cepharadine in plasma by high-performance liquid chromatography using fluorometric detectionJournal of Chromatography B: Biomedical Sciences and Applications, 1983
- Spectrophotometric determination of cephalexin and ampicillin using o-hydroxyhydroquinonephthalein and palladium (II).CHEMICAL & PHARMACEUTICAL BULLETIN, 1982
- High-performance liquid chromatographic determination of cephalexin in human plasma, urine and salivaJournal of Chromatography B: Biomedical Sciences and Applications, 1981
- Determination Of Serum Cephalexin By High Performance Liquid ChromatographyAnalytical Letters, 1981
- Determination of lysine in its cephalexin saltAnalytica Chimica Acta, 1979