Effect of Bile Salts on the Plasma Concentration of Immunoreactive Vasoactive Intestinal Polypeptide in Man.

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Abstract
The effects of bile salts on the release of immunoreactive vasoactive intestinal polypeptide (IR-VIP) were investigated in men using a specific radioimmunoassay. Plasma IR-VIP was determined after extraction by the acid-acetone method (recovery 75 .+-. 5%). Oral administration of 400 mg sodium taurocholate caused a rise in plasma IR-VIP from 18.5 .+-. 1.3 pmol/l to 31.1 .+-. 2.1 pmol/l after 30 min and 39.0 .+-. 1.7 pmol/l after 60 min and return to the initial value after 120 min. Oral administration of chenodeoxycholic acid (CDCA) also increased plasma IR-VIP from a basal level of 14.5 .+-. 1.5 pmol/l to 36.3 .+-. 1.2 pmol/l after 60 min. Oral administration of urosodeoxycholic acid (UDCA) increased plasma IR-VIP from 11.9 .+-. 1.1 pmol/l to 25.6 .+-. 1.8 pmol/l after 30 min. Perifusion of 1 mM taurocholate stimulated release of IR-VIP from human duodenal mucosa into the perifusate. The results suggest that bile salts may participate, at least in part, in the release of IR-VIP from the gut.