INCORPORATION OF [14C] HEXOSES INTO CARBOHYDRATE OF GAMMA2-GLOBULIN IN VITRO

  • 1 January 1965
    • journal article
    • research article
    • Vol. 8  (1) , 49-+
Abstract
A method for the labelling and isolation of specific gamma2-globulin antibody formed in vitro by intact rabbit lymphoid cells has been described. This method has been applied to the study of the synthesis of the carbohydrate portion of gamma2-globulin using [l4C] mannose, [14C] glucose and[14C] galactose as labelled precursors. Metabolic alterations of all three sugars led to redistribution of much of the 14c. label into the amino acids of the protein portion, but a 6- to 18-fold relative enrichment, on a weight basis, was observed in the carbohydrate fraction. Labelling was greatest with mannose and least with galactose under the conditions used in this study.