Cloning of the 62-Kilodalton Component of Basic Transcription Factor BTF2
- 4 September 1992
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 257 (5075) , 1392-1395
- https://doi.org/10.1126/science.1529339
Abstract
Cloning of the mammalian basic transcription factors serves as a major step in understanding the mechanism of transcription initiation. The 62-kilodalton component (p62) of one of these transcription factors, BTF2 was cloned and overexpressed. A monoclonal antibody to this polypeptide inhibited transcription in vitro. Immunoaffinity experiments demonstrated that the 62-kilodalton component is closely associated with the other polypeptides present in the BTF2 factor. Sequence similarity suggests that BTF2 may be the human counterpart of RNA polymerase II initiation factor b from yeast.Keywords
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