Detection of specific anti-leptospiral immunoglobulins M and G in human serum by solid-phase enzyme-linked immunosorbent assay

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Abstract
The enzyme-linked immunosorbent assay (ELISA) was used to detect leptospire-specific IgM and IgG in the sera of patients infected with leptospiral [Leptospira] serovars hardjo, pomona or copenhageni. All patients produced specific IgM and IgG detectable by ELISA. Only a few patients produced IgG agglutinins, whereas all produced IgM agglutinins. The specificity and sensitivity of the test suggest that the ELISA anti-IgM technique is a suitable method for detecting leptospiral antibodies in human sera for diagnostic and epidemiological purposes.