THE DETERMINATION OF α- AND β-ACIDS IN HOPS AND HOP PRODUCTS USING HPLC
Open Access
- 8 July 1981
- journal article
- research article
- Published by Chartered Institute of Brewers and Distillers in Journal of the Institute of Brewing
- Vol. 87 (4) , 225-228
- https://doi.org/10.1002/j.2050-0416.1981.tb04019.x
Abstract
A rapid reversed phase HPLC method for the analysis for α- and β-acids in hops and hop products is described and has been evaluated. The method uses citric acid in the eluent as a complexing agent to overcome the irreversible adsorption effects shown by some columns, thus allowing optimum eluent pH to be selected. The precision of the method for analysis of hop extract has been determined giving relative standard deviations of 1·0% and 2·1% for α- and β-acids respectively. General agreement with results obtained using a polarimetric α-acids analysis method for hop extracts and hops has been demonstrated.Keywords
This publication has 12 references indexed in Scilit:
- HOP RESIN ANALYSIS BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY ON MICROPARTICULATE SILICAJournal of the Institute of Brewing, 1979
- High-performance liquid chromatography of hop bitter substancesJournal of Chromatography A, 1978
- PHOTOMETRIC CONSTANTS OF HOP BITTER ACIDSJournal of the Institute of Brewing, 1978
- THE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY OF HOP RESINSJournal of the Institute of Brewing, 1978
- High-Performance Liquid Chromatography of Hop Compounds in BrewingJournal of the American Society of Brewing Chemists, 1976
- High-pressure liquid chromatography in the separation and detection of bitter compoundsJournal of Agricultural and Food Chemistry, 1973
- AUTOMATIC ANALYSIS OF HOP RESINS BY ION EXCHANGE CHROMATOGRAPHYJournal of the Institute of Brewing, 1972
- Ion-Exchange Chromatographic Assay for Iso-α-Acids in Wort and BeerProceedings. Annual meeting - American Society of Brewing Chemists, 1969
- THE ISOMERIZATION OF HUMULONEJournal of the Institute of Brewing, 1965
- Spectrophotometric Determination of Humulone Complex and Lupulone in HopsAnalytical Chemistry, 1954