Isolation of a Yeast Protein Kinase Gene by Screening with a Mammalian Protein Kinase cDNA
- 1 September 1988
- journal article
- research article
- Published by Mary Ann Liebert Inc in DNA
- Vol. 7 (7) , 469-474
- https://doi.org/10.1089/dna.1.1988.7.469
Abstract
The gene for a new Saccharomyces cerevisiae protein kinase was identified and isolated by hybridization to a cloned cDNA for the catalytic subunit of bovine cAMP-dependent protein kinase. This newly isolated yeast gene encodes a 680-amino-acid protein that shows strong sequence similarity to several serine/threonine protein kinases. The protein kinase catalytic domain is located at the carboxy-terminal portion of the protein. A large, amino-terminal domain shows no sequence similarity to any known protein kinase. It seems likely that the amino-terminal domain mediates the effects of an unknown regulator of the newly identified yeast protein kinase.This publication has 28 references indexed in Scilit:
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