MONOIODOINSULIN SPECIFICALLY SUBSTITUTED IN TYR A14 OR TYR A19
- 1 May 1980
- journal article
- research article
- Published by Wiley in International Journal of Peptide and Protein Research
- Vol. 15 (5) , 495-502
- https://doi.org/10.1111/j.1399-3011.1980.tb02927.x
Abstract
Porcine monoiodoinsulin was prepared using ion exchange chromatography. The isolated monoiodoinsulin showed on polyacrylamide gel electrophoresis 2 bands with different intensities related to the initial method of iodination. Each of the 2 bands were isolated from the gel, and determination of the I distribution among the tyrosyl groups showed that 1 band contained monoiodoinsulin substituted in Tyr A19 contaminated with monoiodoinsulin substituted in the B-chain. The other band contained essentially A14 monoiodoinsulin. Polyacrylamide gel electrophoresis is a convenient method to prepare homogeneous A14 monoindoinsulin with biological activity indistinguishable from that of native insulin.Keywords
This publication has 12 references indexed in Scilit:
- Biological potency and binding affinity of monoiodoinsulin with iodine in tyrosine A14 or tyrosine A19Biochemical and Biophysical Research Communications, 1979
- [127-I]- or carrier-free [125-I]monoiodoinsulinJournal of Biological Chemistry, 1975
- PREPARATION AND CHARACTERIZATION OF MONOIODOINSULINInternational Journal of Peptide and Protein Research, 1974
- Preparation and properties of (monoiodotyrosine)-insulinBiochemical Journal, 1972
- Effect of lodination on the Biological Activity of InsulinHormone and Metabolic Research, 1972
- Monoiodoinsulin: Demonstration of its biological activity and binding to fat cells and liver membranesBiochemical and Biophysical Research Communications, 1971
- THE IMMUNOLOGICAL REACTIVITY AND BIOLOGICAL ACTIVITY OF IODINATED INSULINActa Endocrinologica, 1968
- States of amino acid residues in proteins: VII. Hydrogen bonding of tyrosine residues in the insulin moleculeBiochimica et Biophysica Acta (BBA) - General Subjects, 1965
- The reactivity of the tyrosine residues in insulin with respect to iodine IIRecueil des Travaux Chimiques des Pays-Bas, 1961
- A study of the biological activity of iodinated insulinRecueil des Travaux Chimiques des Pays-Bas, 1961