Regulation by the yeast mating-type locus of STE12, a gene required for cell-type-specific expression.
Open Access
- 1 October 1987
- journal article
- research article
- Published by Taylor & Francis in Molecular and Cellular Biology
- Vol. 7 (10) , 3818-3821
- https://doi.org/10.1128/mcb.7.10.3818
Abstract
The STE12 gene of the yeast Saccharomyces cerevisiae is necessary for RNA synthesis from two sets of cell-type-specific genes. We isolated a recombinant plasmid that carries the STE12 gene by complementation of the mating defect of ste12 cells. The DNA of the cloned gene was used to disrupt the chromosomal STE12 gene and to identify the STE12 transcript. We show that the STE12 transcript level is repressed 5- to 10-fold in a/alpha cells. The STE12 product thus acts to promote diploidy by activating expression of the two sets of genes necessary for mating, and then its synthesis is repressed by products unique to the diploid cell type.This publication has 16 references indexed in Scilit:
- Activation of meiosis and sporulation by repression of the RME1 product in yeastNature, 1986
- The yeast STE12 product is required for expression of two sets of cell-type-specific genesCell, 1985
- Transcription and regulatory signals at the mating type locus in yeastCell, 1984
- [12] One-step gene disruption in yeastPublished by Elsevier ,1983
- Control of yeast cell type by the mating type locusJournal of Molecular Biology, 1981
- A position effect in the control of transcription at yeast mating type lociNature, 1981
- Regulation of transcription in expressed and unexpressed mating type cassettes of yeastNature, 1981
- Mating signals control expression of mutations resulting from insertion of a transposable repetitive element adjacent to diverse yeast genesCell, 1980
- Mutants of Saccharomyces cerevisiae unresponsive to cell division control by polypeptide mating hormone.The Journal of cell biology, 1980
- Transformation in yeast: Development of a hybrid cloning vector and isolation of the can1 geneGene, 1979