Fractionnement du lysozyme par distribution à contre‐courant
- 1 January 1955
- journal article
- research article
- Published by Wiley in Bulletin des Sociétés Chimiques Belges
- Vol. 64 (1-2) , 58-69
- https://doi.org/10.1002/bscb.19550640103
Abstract
The behaviour of the protein enzyme lysozyme towards countercurrent distribution was investigated. Several solvent systems were discarded, owing to poor solubility of the enzyme, denaturation, or/and unfavourable distribution coefficient. A mixture of 2.butanol, water, and p. toluenesulfonic acid was however found efficient.It is established that none of the components of the last mentioned system has inhibiting action upon enzymic activity of the protein. It is further shown that the distribution coefficient of lysozyme is, within limits, a linear function of the acid molarity. This allowed a fractionation of the protein into three components, one of which amounts to nearly 80 %.Keywords
This publication has 13 references indexed in Scilit:
- Separation of the Glycyl from the Phenylalanyl Chain of Oxidized Insulin by Countercurrent Distribution1Journal of the American Chemical Society, 1955
- Partition chromatography of insulin and other proteinsBiochemical Journal, 1953
- Gegenstromverteilung von ProtaminenHoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1953
- The Chemistry of Tyrocidine. I. Isolation and Characterization of a Single PeptideJournal of the American Chemical Society, 1952
- Countercurrent Distribution Studies with InsulinJournal of the American Chemical Society, 1952
- The measurement of lysozyme activity and the ultra-violet inactivation of lysozymeBiochimica et Biophysica Acta, 1952
- Automatic Countercurrent Distribution EquipmentAnalytical Chemistry, 1951
- The chromatographic fractionation of ribonucleaseBiochemical Journal, 1951
- The Binding of Organic Ions by Proteins. Comparison of Native and of Modified ProteinsJournal of the American Chemical Society, 1949
- The Binding of Organic Ions by Proteins. Competition Phenomena and Denaturation EffectsJournal of the American Chemical Society, 1948