Partition chromatography of insulin and other proteins

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Abstract
The prepn. is descr. of liquid 2-phase systems using a variety of glycol ethers, water and organic and inorganic solutes. These systems were found to be suitable for partitioning proteins. Three such systems containing water, ethyl and butyl cellosolves and Na or K phosphate were used for the partition chromatography of insulin. The insulin thus prepared from crude or crystalline material was of uniform activity of about 24 I. U./mg. The recovery from the columns appeared to be near 100% Throughout these chromatographic studies the insulin behaved as a single component. The inherent difficulties of the method and the range of applicability to other proteins are discussed.