The involvement of a ‘bay-region’ and a non-‘bay-region’ diol-epoxide in the metabolic activation of benz(a)anthracene in mouse skin and in hamster embryo cells
- 31 December 1979
- journal article
- research article
- Published by Oxford University Press (OUP) in Carcinogenesis: Integrative Cancer Research
- Vol. 1 (3) , 233-243
- https://doi.org/10.1093/carcin/1.3.233
Abstract
The principal nucleoside-hydrocarbon adducts present in hydrolysates of DNA that were isolated from mouse skin or from hamster embryo cells treated with [the carcinogen] benz[a]anthracene were examined by chromatography on Sephadex LH20 and by high pressure liquid chromatography on Spherisorb 5 ODS. One major adduct prepared from the DNA of mouse skin and of hamster embryo cells has chromatographic properties similar to those of an adduct formed when anti-BA-8,9-diol 10,11-oxide (r-8,t-9-dihydroxy-t-10,11-oxy-8,9,10,11-tetrahydrobenz[a]anthracene) reacts with DNA, whilst a 2nd major adduct has chromatographic properties similar to those of an adduct prepared by reacting anti-BA-3,4-diol 1,2-oxide (t-3,r-4-dihydroxy-t-1,2-oxy-1,2,3,4-tetrahydrobenz[a]anthracene) with DNA. It appears that both of these diol-epoxides may contribute to the biological activity of benz[a]anthracene.This publication has 21 references indexed in Scilit:
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