Sodium Azide Resistance in Proteus hauseri

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Abstract
SUMMARY: The sodium azide resistance of Proteus hauseri is not of the all-or-none unilocal type reported for Escherichia coli but rather of the obligatory multi-step or penicillin variety. Independently isolated 1st-step resistant variants possessed similar degrees of resistance to sodium azide. Some properties of azide-resistant variants of P. hauseri are described. In support of the above finding it was possible to transduce 1st-step (and only 1st-step) resistance into the wild-tpye by phage grown on either 1st-, 2nd-, 3rd- or 4th-step resistant organisms. It was also possible to transduce 2nd-step resistance into 1st-step organisms by phage developed on independently isolated 1st step resistant organisms or by phage from multi-step resistant. About 60 % of transduced genes expressed their phenotype in platings done immediately after the adsorption period. It is concluded that a number of loci, not closely linked, and possibly equipotent, control sodium azide resistance in P. hauseri and that resistance could be dominant to the wild allele.