The Determination of V Factor in the Urine and Tissues of Normal Dogs and of Dogs with Blacktongue by the Use of Hemophilus parainfluenzae

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Abstract
Dogs were maintained on diets deficient in nicotinic acid and diets containing varying amts. of nicotinic acid. The former developed blacktongue. During 2 mos. 1-7 detns. of the amt. of V factor excreted in the urine of 11 dogs were made by interpolation, from the dilution of urine which promoted an amt. of growth of H. parainfluenzae equivalent to that promoted by a known amt. of diphosphopyridine nucleotide (DPN). Growth was measured photometrically. The excretion of V factor was not influenced by intake of nicotinic acid. 8 dogs excreted a fairly equal amount[long dash]0.149 [plus or minus] 0.026 micrograms per cc. Liver, heart and thigh muscle of 3 blacktongue dogs con-tained less V factor than corresponding tissues of 3 normal dogs. Blood remained constant. Liver, kidney and muscle of normal rats contained, respectively, 345, 456 and 353 micrograms per g. DPN equivalent of V factor.