UPTAKE OF CR-51-SRBC IN LOW-RESPONDER AND HIGH-RESPONDER MOUSE STRAINS

  • 1 January 1984
    • journal article
    • research article
    • Vol. 30  (1) , 57-66
Abstract
51Cr-SRBC [sheep red blood cell] antigen clearance was studied in 2 strains of mice differing in the capacity to react with IgG antibody formation. In the B10 strain which is a poor IgG anti SRBC producer, before immunization 80.3% of the injected radioactivity, is taken up by liver; after primary stimulation only 31.1% is taken up. This value decreases further to 22.8% after secondary stimulation. The IgG antibody producing A/J strain accumulates in liver before immunization less antigen than the poorly responding strain (69.8%). In the 10th day after primary immunization there is a higher uptake of the radioactivity in liver than in the poor responder strain (53.8%) and this difference is even more pronounced after the secondary stimulation (49.6%). Interaction between peritoneal macrophages of the B10 and A/J strains before and after immunization with SRBC antigen was assessed by rosette formation. Before immunization the low-responder strain B10 exhibited a 3 times higher level of rosette-forming macrophages, i.e., 6.1% than the high-responder strain A/J (2.0%). After immunization the RFM level in the A/J strain increased 7-fold (13.5%); that in the low-responder strain B10 remained unaffected. A role of macrophage population in regulation of IgG antibody response was suggested.