Correlation between Characteristics of Transformation and Malignancy of Intra and Interspecific Somatic Hybrid Cells

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Abstract
The transformed properties of 5 hybrid cell lines which had 1 or another parent in common were studied and compared with tumorigenicity. Three hybrid cell lines, derived from the Chinese hamster DC-3F/ADX/Aza line, were resistant to actinomycin-D. This property seemed to be correlated with the presence of a marker chromosome from the common parent. Tumorigenicity was intermediate between those of the parent cell lines. Agglutinability by concanavalin A (Con A) was variable. Three hybrid cell lines which had the A9 or the clone 1D (derived from mouse fibroblasts) showed similar transformed characteristics, but 2 were tumorigenic and 1 not so. It appears from this study that the properties of the hybrid cell lines can be influenced more by 1 parent, depending on the genes retained at chromosome segregation. The limits of Con A agglutination as a characteristic of transformation and the validity of check pouch grafts as a tumorigenicity test for malignant human cell lines are discussed.