Epidermal growth factor stimulates the phosphorylation of pyruvate kinase in fleshly isolated rat hepatocytes
- 11 March 1991
- journal article
- Published by Wiley in FEBS Letters
- Vol. 280 (1) , 37-40
- https://doi.org/10.1016/0014-5793(91)80198-c
Abstract
Epidermal growth factor (EGF) has previously been shown to stimulate gluconeogenesis in rat liver by decreasing the activity of pyruvate kinase [(1988) Biochem. J. 255, 361–364]. Here we investigate the mechanism underlying the inactivation of the enzyme. EGF was found to increase the incorporation of phosphate into pyruvate kinase, with maximal phosphorylation achieved only after 10 min in the presence of the growth factor. The increase in phosphorylation was not additive with the caused by cyclic AMP. Phosphoamino acid analysis of pyruvate kinase isolated from cells treated with EGF indicated that EGF phosphorylation solely on serine residues. The exact site of EGF-mediated phosphorylation has yet to be identified.Keywords
This publication has 11 references indexed in Scilit:
- Epidermal growth factor and cyclic AMP stimulate Na+/H+ exchange in isolated rat hepatocytesEuropean Journal of Biochemistry, 1990
- Studies on the autophosphorylation of the insulin receptor from human placenta. Analysis of the sites phosphorylated by two-dimensional peptide mappingBiochemical Journal, 1988
- Epidermal growth factor and angiotensin II stimulate formation of inositol 1,4,5- and inositol 1,3,4-trisphosphate in hepatocytes. Differential inhibition by pertussis toxin and phorbol 12-myristate 13-acetate.Journal of Biological Chemistry, 1987
- Epidermal growth factor, like glucagon, exerts a short-term stimulation of alanine transport in rat hepatocytesBiochemical Journal, 1987
- Kinetics of epidermal growth factor binding and processing in isolated intact rat hepatocytesEuropean Journal of Biochemistry, 1987
- Epidermal growth factor mimics insulin effects in rat hepatocytesBiochemical Journal, 1986
- Phosphorylation of liver pyruvate kinase by Ca++ /calmodulin-dependent protein kinase: Characterization of two phosphorylation sitesBiochemical and Biophysical Research Communications, 1986
- Complete amino acid sequence of rat L-type pyruvate kinase deduced from the cDNA sequenceEuropean Journal of Biochemistry, 1986
- Both insulin and epidermal growth factor stimulate fatty acid synthesis and increase phosphorylation of acetyl‐CoA carboxylase and ATP‐citrate lyase in isolated hepatocytesFEBS Letters, 1985
- Acute regulation of pyruvate kinase activity in rat epididymal adipose tissue by insulinBiochemical Journal, 1979