Sequence and characterization of pvuIIR, the PvuII endonuclease gene, and of pvuIIC, its regulatory gene
- 1 May 1992
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 174 (10) , 3395-3398
- https://doi.org/10.1128/jb.174.10.3395-3398.1992
Abstract
An open reading frame partially overlaps pvuIIR, and genetic evidence implies that this open reading frame, named pvuIIC, specifies a positive regulator of pvuIIR (T. Tao, J. C. Bourne, and R. M. Blumenthal, J. Bacteriol. 173:1367-1375, 1991). Inducible constructs of pvuIIC produced a protein of the expected size. The site of C.PvuII action appears to lie within pvuIIC itself; thus, pvuIIC may be a self-contained regulatory cassette.Keywords
This publication has 16 references indexed in Scilit:
- RESTRICTION AND MODIFICATION SYSTEMSAnnual Review of Genetics, 1991
- Organization of restriction-modification systemsNucleic Acids Research, 1991
- Characterization of the cloned BamHI restriction modification system: its nucleotide sequence, properties of the methylase, and expression in heterologous hostsNucleic Acids Research, 1991
- Complete nucleotide sequence of thePvuii restriction enzyme gene fromProteus vulgarisNucleic Acids Research, 1990
- Nucleotide sequence and genetic organization of the NgoPII restriction-modification system of Neisseria gonorrhoeaeMolecular Genetics and Genomics, 1989
- Interaction of AluI, Cfr6I and PvuII restriction-modification enzymes with substrates containing either N4-methylcytosine or 5-methylcytosineBiochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1987
- A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes.Proceedings of the National Academy of Sciences, 1985
- Two new restriction endonucleases from Proteus vulgarisNucleic Acids Research, 1981
- The sequence of the ribosomal 16S RNA from Proteus vulgaris. Sequence comparison with E. coli 16S RNA and its use in secondary structure model buildingNucleic Acids Research, 1981
- DNA sequencing with chain-terminating inhibitorsProceedings of the National Academy of Sciences, 1977