CRYOPRESERVATION OF HUMAN CADAVER CORNEAS REGENERATED AT 31°N A MODIFIED TISSUE CULTURE MEDIUM
- 31 January 1981
- journal article
- research article
- Published by Wiley in Acta Ophthalmologica
- Vol. 59 (1) , 142-148
- https://doi.org/10.1111/j.1755-3768.1981.tb06722.x
Abstract
Human corneas were obtained 2-82 h post mortem, cultured for 20-28 h in a modified tissue culture medium, frozen at a controlled rate and thawed rapidly. The thawed corneas were subjected to 20-28 h of additional culture. Immediately after thawing, a mean endothelial cell damage of 11% was indicated by trypane blue staining. The mean endothelial cell loss during the subsequent culture was 34%. This cell loss was not related to post mortem time, to donor age, to cell loss during the primary culture or to endothelial cell density.Keywords
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