ULTRASTRUCTURAL AND FUNCTIONAL ASPECTS OF FROG HEART LESIONS AFTER ISOPROTERENOL

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Abstract
The possible correlation between the ultrastructural changes and the functional changes induced by isoproterenol (IPR) in the myocardium of the frog (Rana pipiens) was studied. The experimental animals were moved from +8.degree. C to +25.degree. C, kept there for 3 days and then given 2 daily doses of IPR (200 mg/kg body wt). After the 2nd IPR dose (24 h), specimens were taken from the heart ventricle and subjected to EM examination and to analysis of the isometric force decay in response to anoxia produced by sodium cyanide. Specimens were taken from macroscopically non-damaged portions of the ventricle and from aneurysms. Control animals were kept at +8.degree. C and +25.degree. C and injected with saline. In all IPR-injected animals the specimens from macroscopically non-damaged parts of the myocardium showed ultrastructural changes characterized by a relative increase in mitochondrial mass and a pronounced reduction of glycogen. Specimens from aneurysms showed similar changes but in some fibers signs of more severe injury were observed such as fragmentation of the mitochondrial cristae and disintegration of myofibrils. The functional analyses showed that the isometric force decay of the heart muscle strips induced by the respiratory block by cyanide was much greater in specimens from IPR-treated animals than in those from control frogs. The augmented force decay was found in strips from macroscopically normal parts of the myocardium and in those from aneurysms although it was somewhat greater and more rapid in the latter. The observed, minor functional difference between macroscopically non-damaged portions of the myocardium and aneurysms is remarkable with respect to the different preservation of myofilaments in these regions. This similarity in functional decline could possibly be due to the observed homogeneous reduction of glycogen in the IPR-exposed myocardium since glycogen was the only source of ATP under the current experimental conditions.