Identification of Glutathione Conjugate Formed from Loxistatin in Rats
- 1 January 1989
- journal article
- research article
- Published by Taylor & Francis in Xenobiotica
- Vol. 19 (5) , 521-529
- https://doi.org/10.3109/00498258909042291
Abstract
1. The metabolic fate of loxistatin, ethyl-(+)-(2S,3S)-3-[(S)-3-methyl-1-(3-methyl-butulcarbamoyl)butylcarbamoyl]-2-oxiranecarboxylate, was studied in rats. 2. Following oral administration of 14C-loxistatin the major part of radioactivity was eliminated via the bile into the faeces. 3. The major metabolites in bile were isolated and identified as glutathione and cysteine conjugates of loxistatin acid, by chromatography, proton n.m.r. spectroscopy and mass spectrometry and comparison with those of the authentic reference compounds. 4. Formation of the glutathione conjugate was also demonstrated in vitro by incubation of 14C-loxistatin acid with rat liver cytosol and glutathione.This publication has 19 references indexed in Scilit:
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