Isolation and structure of theStreptococcus faecalissex pheromone, cAM373
Open Access
- 29 September 1986
- journal article
- Published by Wiley in FEBS Letters
- Vol. 206 (1) , 69-72
- https://doi.org/10.1016/0014-5793(86)81342-4
Abstract
The Streptococcus faecalis sex pheromone, cAM373, which induces a mating response of donor cells harboring plasmid pAM373 and is also produced by Staphylococcus aureus, was isolated and its structure determined. Supernatant from an overnight culture of a recipient strain was subjected to successive purification procedures, and 4.4 μg cAM373 was obtained. The isolated pheromone showed activity at a concentration as low as 5 × 10−11 M. Sequence analysis indicated that cAM373 was a heptapeptide, H‐Ala‐Ile‐Phe‐Ile‐Leu‐Ala‐Ser‐OH, and that its M r was 733. A synthetic replicate of the peptide showed the same biological activity and chromatographic behavior as the native cAM373.Keywords
This publication has 5 references indexed in Scilit:
- Isolation and structure of the bacterial sex pheromone, cAD1, that induces plasmid transfer in Streptococcus faecalisFEBS Letters, 1984
- Isolation and Structure of Bacterial Sex Pheromone, cPD1Science, 1984
- Modification of Streptococcus faecalis sex pheromones after acquisition of plasmid DNA.Proceedings of the National Academy of Sciences, 1983
- Plasmid transfer in Streptococcus faecalis: Production of multiple sex pheromones by recipientsPlasmid, 1979
- The use of N-hydroxy-5-norbornene-2,3-dicarboximide acitive esters in peptide synthesis.CHEMICAL & PHARMACEUTICAL BULLETIN, 1974