Calcium binding to bone gamma-carboxyglutamic acid protein from calf studied by 43Ca NMR
- 1 July 1986
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 158 (2) , 373-376
- https://doi.org/10.1111/j.1432-1033.1986.tb09762.x
Abstract
Calcium binding to bone .gamma.-carboxyglutamic acid protein (BCB) from calf has been studied using 43Ca NMR. The temperature dependence of the 43Ca NMR signal has been used to calculate the calcium ion exchange rate, koff. The dependence of the 43Ca NMR band shape on the [Ca2+]/[BGP] ratio fits well to a chemical equilibrium model having a single Ca2+-binding site with an association constant in the range of 5 .times. 103-1 .times. 105 M-1. The pH dependence of the 43Ca NMR line-width shows a single apparent pKa value of 5.1.This publication has 25 references indexed in Scilit:
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