Studies on transfer ribonucleic acids and related compounds. XXXI. Synthesis of the 3',5'-bisphosphorylated hexanucleotide corresponding to bases (72-77) of tRNAfMet from E. coli and its base transition analog.
- 1 January 1980
- journal article
- research article
- Published by Pharmaceutical Society of Japan in CHEMICAL & PHARMACEUTICAL BULLETIN
- Vol. 28 (1) , 120-125
- https://doi.org/10.1248/cpb.28.120
Abstract
A hexanucleotide pC-A-A-C-C-Ap, which corresponds to the 3''-end of [Escherichia coli] tRNAfMet, was synthesized as a bisphosphorylated form, which should be a suitable donor substrate for joining with RNA ligase. 3''-Phosphorylated oligonucleotides were condensed by a triester method and the 5''-terminus of the protected hexanucleotide was phosphorylated with dianilidophosphorochloridate after demonomethoxytritylation. A site-directed base transition mutant sequence C-G-A-C-C-Ap was also synthesized by the triester method using suitably protected 3''-phosphorylated oligonucleotides.This publication has 7 references indexed in Scilit:
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